Journal: Frontiers in Nutrition

Article Title: High intake of n-6 polyunsaturated fatty acid exacerbates non-alcoholic steatohepatitis by the involvement of multiple metabolic pathways

doi: 10.3389/fnut.2025.1562509

Figure Lengend Snippet: Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating CD163-positive cells, green arrows showing CD68-positive cells, and yellow arrows marking CD163 and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.

Article Snippet: BCA protein quantitation kit (Boster Bio, Cat # AR0146), RIPA lysis buffer (Boster Bio, Cat # 0105), protease inhibitor cocktails (Boster, Bio Cat # AR1182), phosphatase inhibitor (Boster Bio, Cat # AR1183), color pre-dyed protein marker (Boster Bio, Cat # AR1113), Western-specific primary and secondary antibody diluent (Boster Bio, Cat # AR1017), wash buffer TBS-T (Boster Bio, Cat # AR0195-10), ECL chemiluminescent reagent (Boster Bio, Cat # AR1196), BSA TBS buffer system blocking solution (Boster Bio, Cat # AR0189), NF-κB antibody (Boster Bio, Cat # A01228-1), GAPDH antibody (Boster Bio, Cat # M00227), HRP-conjugated goat anti-rabbit IgG (Boster Bio, Cat # BA1054), CD163 antibody (Boster Bio, Cat # A00812-2), CD11c antibody (Boster Bio, Cat # A00357-3), CD68 antibody (Boster Bio, Cat # BA3638), fluorescent (DyLight 488) labeled goat anti-rabbit IgG (Boster Bio, Cat # BA1127), fluorescent (DyLight 594) labeled goat anti-rabbit IgG (Boster Bio, Cat # BA1142), rat TNF-α ELISA kit (Jiangsu Enzyme Immunoassay Co., Ltd., Cat # MM-0180R1), rat IL-1β ELISA kit (Boster Bio, Cat # EK0393), rat IL-4 ELISA kit (Jiangsu Enzyme Immunoassay Co., Ltd., Cat # MM-0191R1), rat IL-10 ELISA kit (Boster Bio, Cat # EK0418), 25 VD3 assay kit (Roche Diagnostics, Cat # 07028148190), TAOC assay kit (Nanjing Jiancheng, Cat # A015-3-1), MDA assay kit (Nanjing Jiancheng, Cat # A003-1-2), and free fatty acid kit (Kunchuang Biotechnology, Xian, China, Cat # SK125-2).

Techniques: Double Staining, Expressing

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy

doi:

Figure Lengend Snippet: Oligonucleotide primers of CD11, CD11b, CD11c, and β-actin

Article Snippet: Primary antibodies of CD11a, CD11b, and CD11c (1:100, Wuhan Boster Company) were incubated overnight at 4°C and then incubated at 37°C for 30 min in IgG/Biotin IgG and SABC liquid.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy

doi:

Figure Lengend Snippet: RT-PCR products of CD11a, CD11b, and CD11c A: RT-PCR products of CD11a M: Marker 1-2: experimental group; 3-4: control group B: RT-PCR products of CD11b M: Marker 1-2: control group; 3-4: experimental group C: RT-PCR products of CD11c M: Marker 1-2: experimental group; 3-4: control group

Article Snippet: Primary antibodies of CD11a, CD11b, and CD11c (1:100, Wuhan Boster Company) were incubated overnight at 4°C and then incubated at 37°C for 30 min in IgG/Biotin IgG and SABC liquid.

Techniques: Reverse Transcription Polymerase Chain Reaction, Marker, Control

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy

doi:

Figure Lengend Snippet: Comparison of the average area and brightness of CD11a, CD11b, and CD11c expressions in the myocardium ( x ¯ ±s)

Article Snippet: Primary antibodies of CD11a, CD11b, and CD11c (1:100, Wuhan Boster Company) were incubated overnight at 4°C and then incubated at 37°C for 30 min in IgG/Biotin IgG and SABC liquid.

Techniques: Comparison, Control

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy

doi:

Figure Lengend Snippet: Expressions of CD11a, CD11b, and CD11c in the myocardial tissue at mRNA levels A: CD11a of control group B: CD11b of control group C: CD11c of control group D: CD11a of experimental group E: CD11b of experimental group F: CD11c of experimental group Arrows was used to indicate the positive expression of integrins in experimental group.

Article Snippet: Primary antibodies of CD11a, CD11b, and CD11c (1:100, Wuhan Boster Company) were incubated overnight at 4°C and then incubated at 37°C for 30 min in IgG/Biotin IgG and SABC liquid.

Techniques: Control, Expressing